Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

General Botulinum Toxin F (BTX-F)ELISA Kit

SKU Code LBEK-002G
Species General
Size 96 T | 48 T
Price POR
SKU: LBEK-002G
General Botulinum Toxin A (BTX-A)ELISA Kit
Sample Volume 10ul
Wavelength 450nm
Purpose Our General Botulinum Toxin F (BTX-F) ELISA Kit is to for the qualitative determination of BTX-F in General serum, plasma, culture media or any biological fluid.

Additional information

sensitivity

detection-range

Brand

Link Biotech

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to BTX-F, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for BTX-F is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain BTX-F and HRP conjugated BTX-F antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of BTX-F is determined by comparing with the CUTOFF value.