MF Rat EGF ELISA Kit | Link Biotech

MF-Rat EGF(Epidermal Growth Factor) ELISA Kit
Uniprot ID	P07522
Detection Method	Sandwich
Standard	1000pg/mL
Reaction time	3hours
Sample Type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Research Area	Signaling transduction, Cell biology, Cancer, Developmental Biology;
Alternative Names	URG, HOMG4, Beta-Urogastrone
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat EGF. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the OD of the samples to the standard curve.

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