MF-Rat cTnT/TNNT2 ELISA Kit

MF-Rat cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Uniprot ID	P50753
Detection Method	Sandwich
Standard	1000pg/mL
Reaction time	3hours
Sample Type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Research Area	Cardiovascular, Signaling transduction, Cancer, Stem Cells;
Alternative Names	CMH2, CMPD2, LVNC6, RCM3, TnTC
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Rat cTnT/TNNT2. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.

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