Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

MF-Mouse S100B ELISA Kit

SKU Code LBEK-0050M
Species Mouse
Sensitivity 46.88 pg/mL
Detection Range 78.13-5000pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0050M
MF-Mouse S100B(S100 Calcium Binding Protein B) ELISA Kit
Uniprot ID P50114
Detection Method Sandwich
Standard 5000pg/mL
Reaction time 3hours
Sample Type Serum, Plasma and Tissue homogenate and Other biological samples;Sample volume:25μL
Research Area Cell biology, Epigenetics and Nuclear Signaling, Cancer, Stem Cells;
Alternative Names S100-B, NEF, S100Beta
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse S100B. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse S100B. You can calculate the concentration of Mouse S100B in the samples by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

46.88 pg/mL

detection-range

78.13-5000pg/mL

brand

Link Biotech