MF-Human EGF ELISA Kit

MF-Human EGF(Epidermal Growth Factor) ELISA Kit
Uniprot ID	P01133
Detection Method	Sandwich
Standard	100pg/mL
Reaction time	3hours
Sample Type	Serum, Plasma; Sample volume：25μL
Research Area	Signaling transduction, Cell biology, Cancer, Developmental Biology;
Alternative Names	URG, HOMG4, Beta-Urogastrone
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human EGF. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human EGF. You can calculate the concentration of Human EGF in the samples by comparing the OD of the samples to the standard curve.

Additional information