Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

MF-Mouse LIF ELISA Kit

SKU Code LBEK-0044M
Species Mouse
Sensitivity 5.54 pg/mL
Detection Range 7.81-500pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0044M
MF-Mouse LIF(Leukemia Inhibitory Factor) ELISA Kit
Uniprot ID P09056
Detection Method Sandwich
Standard 500pg/mL
Reaction time 3hours
Sample Type Serum, Plasma and Tissue homogenate and Other biological samples;Sample volume:25μL
Research Area CD & Adhesion molecule;Tumor immunity;Infection immunity;
Alternative Names CDF, D Factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, leukemia inhibitory factor (cholinergic differentiation factor), leukemia inhibitory factor, LIF, Melanoma-derived LPL inhibitor, MLPLI
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIF. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse LIF. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse LIF. You can calculate the concentration of Mouse LIF in the samples by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

5.54 pg/mL

detection-range

7.81-500pg/mL

brand

Link Biotech