MF-Mouse PD-1 ELISA Kit

MF-Mouse PD-1(Programmed Cell Death Protein 1) ELISA Kit
Uniprot ID	Q02242
Detection Method	Sandwich
Standard	1000pg/mL
Reaction time	3hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=25μL
Research Area	Cardiovascular, Cancer, Immunology
Alternative Names	CD279 antigen, CD279, hPD-1, PD1, PD-1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PD-1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse PD-1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PD-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse PD-1. You can calculate the concentration of Mouse PD-1 in the samples by comparing the OD of the samples to the standard curve.

Additional information