MF-Mouse TSH ELISA Kit

MF-Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit
Uniprot ID	P12656
Detection Method	Sandwich
Standard	80ng/mL
Reaction time	2.5hours
Sample Type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=25μL
Research Area	Metabolic pathway;Endocrinology;Hormone metabolism;
Alternative Names	Thyrotropin
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.

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