FS-ALD ELISA Kit

FS-ALD(Aldosterone) ELISA Kit
Uniprot ID	N/A
Detection Method	Competitive
Standard	2000pg/mL
Reaction time	45min
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Alternative Names	Electrocortin‌,Aldocorten‌,Aldocortene‌,Aldocortin‌,Reichstein X‌,18-Oxocorticosterone‌,NSC 73856
Test Principle	This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cort. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Cort. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

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