Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-E2 ELISA Kit

SKU Code LBEK-0010U
Species Universal
Sensitivity 11.25 pg/mL
Detection Range 12.5-800pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0010U
FS-E2(Estradiol) ELISA Kit
Uniprot ID N/A
Detection Method Competitive
Standard 800pg/mL
Reaction time 45min
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Alternative Names Oestradiol, E2, 17β-Estradiol, Estra-1,3,5(10)-triene-3,17β-diol
Test Principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

11.25 pg/mL

detection-range

12.5-800pg/mL

brand

Link Biotech