FS-Mouse IL-6R ELISA Kit

FS-Mouse IL-6R(Interleukin 6 Receptor) ELISA Kit
Uniprot ID	P22272
Detection Method	Sandwich
Standard	2500pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cardiovascular, Neuroscience, Cancer, Immunology
Alternative Names	CD126, gp80, IL-6 R alpha, IL6Q, IL6R alpha, IL-6R alpha, IL6R, IL-6R-1, IL6RA, IL-6Ra, IL6RQ, interleukin 6 receptor
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6R. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-6R are added to the micro ELISA plate wells. Mouse IL-6R in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-6R. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IL-6R. The concentration of Mouse IL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

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