FS-Human CYFRA21-1 ELISA Kit

FS-Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit
Uniprot ID	P08727
Detection Method	Sandwich
Standard	40ng/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	KRT19, CK19, K19, K1CS, keratin 19
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CYFRA21-1 are added to the micro ELISA plate wells. Human CYFRA21-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CYFRA21-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CYFRA21-1. The concentration of Human CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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