FS-Mouse LEP ELISA Kit

FS-Mouse LEP(Leptin) ELISA Kit
Uniprot ID	P41160
Detection Method	Sandwich
Standard	5000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Neuroscience, Signaling transduction, Cancer;
Alternative Names	FLJ94114, LEP, leptin (murine obesity homolog), leptin (obesity homolog, mouse), Leptin, OB, Obese protein, obese, mouse, homolog of, Obesity factor, OBOBS
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse LEP are added to the micro ELISA plate wells. Mouse LEP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse LEP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse LEP. The concentration of Mouse LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

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