Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Human IGFBP-1 ELISA Kit

SKU Code LBEK-0033H
Species Human
Sensitivity 0.09 ng/mL
Detection Range 0.16-10ng/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0033H
FS-Human IGFBP-1( insulin-like growth factors binding protein 1) ELISA Kit
Uniprot ID P08833
Detection Method Sandwich
Standard 10ng/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Cancer, Immunology;
Alternative Names alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, hIGFBP-1, IBP1, IBP-1, IGF-binding protein 1, IGFBP1, IGFBP-1, IGF-BP25, insulin-like growth factor binding protein 1, insulin-like growth factor-binding protein 1, Placental protein 12, PP12AFBP
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGFBP-1 are added to the micro ELISA plate wells. Human IGFBP-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGFBP-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IGFBP-1. The concentration of Human IGFBP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.09 ng/mL

detection-range

0.16-10ng/mL

Brand

Link Biotech