Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Human NSE ELISA Kit

SKU Code LBEK-0032H
Species Human
Sensitivity 15.63 pg/mL
Detection Range 78.13-5000 pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0032H
FS-Human NSE(Neuron Specific Enolase) ELISA Kit
Uniprot ID P09104
Detection Method Sandwich
Standard 5000pg/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Cancer, Immunology;
Alternative Names 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, ENO2, enolase 2 (gamma, neuronal), Enolase 2, gamma-Enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuronspecific Enolase, Neuron-specific enolase, NSE
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human NSE are added to the micro ELISA plate wells. Human NSE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human NSE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human NSE. The concentration of Human NSE in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

15.63 pg/mL

detection-range

78.13-5000 pg/mL

Brand

Link Biotech