FS-Human PDGF-BB ELISA Kit

FS-Human PDGF-BB(Platelet Derived Growth Factor BB) ELISA Kit
Uniprot ID	P01127(PDGF-B)
Detection Method	Sandwich
Standard	2000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	c-sis,IBGC5,PDGF2,PDGF-2,PDGFB,Platelet-derived growth factor subunit B,SIS,SSV, PDGFBB, PDGF-BB
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PDGF-BB are added to the micro ELISA plate wells. Human PDGF-BB in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PDGF-BB. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human PDGF-BB. The concentration of Human PDGF-BB in the samples is then determined by comparing the OD of the samples to the standard curve.

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