FS-Human CA242 ELISA Kit

FS-Human CA242(Pancreatic Carcinoma Markers-CA242) ELISA Kit
Uniprot ID	N/A
Detection Method	Sandwich
Standard	10IU/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	CA242, Cancer Antigen 242, Carbohydrate Antigen 242
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA242. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CA242 are added to the micro ELISA plate wells. Human CA242 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CA242. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CA242. The concentration of Human CA242 in the samples is then determined by comparing the OD of the samples to the standard curve.

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