Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Human IL-6 ELISA Kit

SKU Code LBEK-0028H
Species Human
Sensitivity 0.81 pg/mL
Detection Range 1.56-100pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0028H
FS-Human IL-6(Interleukin 6) ELISA Kit
Uniprot ID P05231
Detection Method Sandwich
Standard 100pg/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Signaling transduction, Cancer;
Alternative Names B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.81 pg/mL

detection-range

1.56-100pg/mL

Brand

Link Biotech