Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Human CCL5/RANTES ELISA Kit

SKU Code LBEK-0026H
Species Human
Sensitivity 0.42 pg/mL
Detection Range 3.12-200pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0026H
FS-Human CCL5/RANTES(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
Uniprot ID P13501
Detection Method Sandwich
Standard 200pg/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Signaling transduction, Cancer;
Alternative Names CCL5, chemokine (C-C motif) ligand 5, D17S136Enormally T-expressed, and presumably secreted, EoCP, Eosinophil chemotactic cytokine, RANTES, SISd, SIS-delta, small inducible cytokine A5 (RANTES), small inducible cytokine subfamily A (Cys-Cys), member 5, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, TCP228T-cell-specific protein RANTES
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL5/RANTES. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CCL5/RANTES are added to the micro ELISA plate wells. Human CCL5/RANTES in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CCL5/RANTES. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CCL5/RANTES. The concentration of Human CCL5/RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.42 pg/mL

detection-range

3.12-200pg/mL

Brand

Link Biotech