FS-Human 5-NT ELISA Kit

FS-Human 5-NT(5-Nucleotidase) ELISA Kit
Uniprot ID	P21589
Detection Method	Sandwich
Standard	2000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	5' nucleotidase (CD73), 5'-NT, 5-NT, 5'-Nucleotidase, 5'-nucleotidase, ecto (CD73), CD73 antigen, CD73, E5NT, EC 3.1.3.5, Ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NT5E, NTE, Purine 5-Prime-Nucleotidase
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human 5-NT are added to the micro ELISA plate wells. Human 5-NT in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human 5-NT. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human 5-NT. The concentration of Human 5-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

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