FS-Human IgE ELISA Kit

FS-Human IgE(Immunoglobulin E) ELISA Kit
Uniprot ID	N/A
Detection Method	Sandwich
Standard	20ng/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	IgE
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgE are added to the micro ELISA plate wells. Human IgE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IgE. The concentration of Human IgE in the samples is then determined by comparing the OD of the samples to the standard curve.

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