Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-T4 ELISA Kit

SKU Code LBEK-009U
Species Universal
Sensitivity 0.23 ng/mL
Detection Range 0.39-25ng/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-009U
FS-T4(Thyroxine) ELISA Kit
Uniprot ID N/A
Detection Method Competitive
Standard 25ng/mL
Reaction time 45min
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Cancer, Immunology;
Alternative Names FTI
Test Principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T4. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to T4. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.23 ng/mL

detection-range

0.39-25ng/mL

Brand

Link Biotech