FS-Human IGFBP-3 ELISA Kit

FS-Human IGFBP-3(Insulin-like Growth Factor Binding Protein 3) ELISA Kit
Uniprot ID	P17936
Detection Method	Sandwich
Standard	10ng/mL
Reaction time	1.5 hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	IGFBP3, BP-53, IBP3
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP-3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGFBP-3 are added to the micro ELISA plate wells. Human IGFBP-3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGFBP-3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IGFBP-3. The concentration of Human IGFBP-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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