Mouse LIF(Leukemia Inhibitory Factor) ELISA Kit

Mouse LIF(Leukemia Inhibitory Factor) ELISA Kit
Uniprot ID	P09056
Detection Method	Sandwich
Standard	50pg/mL
Reaction time	3.5 hours
Sample Type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Research Area	CD & Adhesion molecule;Tumor immunity;Infection immunity;
Alternative Names	CD & Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LIF. You can calculate the concentration of Mouse LIF in the samples by comparing the OD of the samples to the standard curve.

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