COVID-19 Neutralizing Antibody Elisa Kit

SKU Code LBFN-063U
Species Universal
Sensitivity 0.938ng/ml
Detection Range 1.563-100ng/ml
Size 96 T | 48 T
Price POR
SKU: LBFN-063U
COVID-19 Neutralizing Antibody ELISA Kit - Details
Alternative Names Neutralizing antibody ELISA Kit
Detection Wavelength OD450
Detection Method Sandwich ELISA, Double Antigen
Sample Type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Reaction Duration 4 Hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with COVID-19 Neutralizing Antibody , no obvious cross reaction with other analogues.

Additional information

Sensitivity

0.938ng/ml

Detection-Range

1.563-100ng/ml

This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with COVID-19 Neutralizing Antibody bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of COVID-19 Neutralizing Antibody in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.