Human anti-SARS-CoV2(N) IgG Elisa Kit

SKU Code LBFN-060H
Species Human
Sensitivity 2.344ng/ml
Detection Range 3.906-250ng/ml
Size 96 T | 48 T
Price POR
SKU: LBFN-060H
Human anti-SARS-CoV2(N) IgG ELISA Kit - Details
Alternative Names SARS-CoV-2(N) IgG ELISA Kit
Detection Wavelength OD450
Detection Method Indirect ELISA
Sample Type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Reaction Duration 1.5 Hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with anti-SARS-CoV2 (N) IgG , no obvious cross reaction with other analogues.

Additional information

Sensitivity

2.344ng/ml

Detection-Range

3.906-250ng/ml

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-SARS-CoV2 (N) IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-SARS-CoV2 (N) IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.