| Human AQP-4(Aquaporin-4) ELISA Kit - Details | |
| Alternative Names | Aquaporin-4 ELISA Kit, AQP-4 ELISA Kit, Mercurial-insensitive water channel ELISA Kit, MIWC ELISA Kit, WCH4 ELISA Kit, AQP4 ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 4 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with AQP-4 , no obvious cross reaction with other analogues. |
| UniProt ID | P55087 |
Human AQP-4 (Aquaporin-4) Elisa Kit
| SKU Code | LBFN-040H |
| Species | Human |
| Sensitivity | 0.188ng/ml |
| Detection Range | 0.313-20ng/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 0.188ng/ml |
|---|---|
| Detection Range | 0.313-20ng/ml |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AQP-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with AQP-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of AQP-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.