Link Biotech
E-mail: support@linkbiotech.co.in
Website: www.linkbiotech.co.in

Enfortumab(anti-NECTIN4) Elisa Kit

SKU Code LBFN-032U
Species Universal
Sensitivity 0.188ng/ml
Detection Range 0.313-20ng/ml
Size 96 T | 48 T
Price POR
SKU: LBFN-032U
Enfortumab(anti-NECTIN4) ELISA Kit - Details
Alternative Names Enfortumab ELISA Kit, anti-NECTIN4 ELISA Kit
Sample Type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Wavelength OD450
Detection Method Indirect ELISA
Reaction Duration 4 hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with Enfortumab, no obvious cross reaction with other analogues.

Additional information

Sensitivity

0.188ng/ml

Detection Range

0.313-20ng/ml

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Enfortumab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Enfortumab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.