The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Molecular Weight Estimation (kDa)
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That’s why we’re pleased to launch the color-enhanced Blu10 Plus prestained protein standards. As you may see the comparison data shown below, Blu10 Plus shows a better resolution than the Blu12 & Blu10 for the protein bands from 95 kDa to 270 kDa.
Storage Buffer
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Storage
- Stable for up to 2 weeks at 25°C.
- Stable for up to 3 months at 4°C.
- Stable for up to 24 months at -20°C.
