Link Biotech

Elisa-Kit

Human INS(Insulin) ELISA Kit

SKU Code LBRB-014H
Species Human
Sensitivity 0.95 μIU/mL
Detection Range 1.56-100μIU/mL
Size 96 T | 48 T
Price $ 365 | $ 265
SKU: LBRB-014H
Categories: ,

Description

Human INS(Insulin) ELISA Kit - Product Details
Alternative Names IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin
Uniprot ID P01308
Standard 100μIU/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time 3.5H
Research Area Enzyme & Kinase
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the OD of the samples to the standard curve.

Additional information

Sensitivity

0.95 μIU/mL
Detection Range

1.56-100μIU/mL

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
100.00 2.504 2.435
50.00 1.648 1.579
25.00 0.982 0.913
12.50 0.463 0.394
6.25 0.254 0.185
3.13 0.156 0.087
1.56 0.104 0.035
0.00 0.069 0.000

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(μIU/mL) 0.81 11.01 20.69 1.06 8.17 24.16
Standard deviation 0.06 0.59 0.92 0.05 0.58 0.82
C V (%) 3.17 4.75 6.26 3.36 5.93 4.33

Rate of Recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Avg Recovery(%)
Serum (n=8) 80-94 87
EDTA plasma (n=8) 89-101 95
Cell culture media (n=8) 82-95 88

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-102 88-97 95-102
Average (%) 98 93 96
1:4 Range (%) 80-92 86-92 87-101
Average (%) 81 90 101
1:8 Range (%) 87-98 95-103 82-91
Average (%) 97 97 90
1:16 Range (%) 80-93 98-106 85-97
Average (%) 80 104 96

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