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TWO-DIMENSIONAL GEL ELECTROPHORESIS

Two-dimensional gel electrophoresis is combined with isoelectric focusing (IEF) and SDS-PAGE methods to enhance the resolution of the electrophoresis.

The separation of different proteins with two-dimensional gel electrophoresis is based on the isoelectric point and the molecular point of different proteins. With two different separating methods, we are able to identify and quantify thousands of proteins.



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